Human enteric Caliciviridae: a new prevalent small round-structured virus group defined by RNA-dependent RNA polymerase and capsid diversity
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منابع مشابه
Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
متن کاملHuman enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus.
Comparisons of the RNA polymerase and capsid sequences of small round structured viruses (SRSVs) have recently shown these are genetically diverse viruses which fall into two distinct groups. The genomes of two group I viruses, Southampton and Norwalk viruses have been characterized; however, similar data for the genetic group II SRSVs have not been available until now. We report here the compl...
متن کاملenhancement of rna interference effect in p19 ec cells by an rna-dependent rna polymerase
background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...
متن کاملTemplate requirements for RNA synthesis by a recombinant hepatitis C virus RNA-dependent RNA polymerase.
The RNA-dependent RNA polymerase (RdRp) from hepatitis C virus (HCV), nonstructural protein 5B (NS5B), has recently been shown to direct de novo initiation using a number of complex RNA templates. In this study, we analyzed the features in simple RNA templates that are required to direct de novo initiation of RNA synthesis by HCV NS5B. NS5B was found to protect RNA fragments of 8 to 10 nucleoti...
متن کاملIdentification of a Pyridoxine-Derived Small-Molecule Inhibitor Targeting Dengue Virus RNA-Dependent RNA Polymerase.
The viral RNA-dependent RNA polymerase (RdRp) activity of the dengue virus (DENV) NS5 protein is an attractive target for drug design. Here, we report the identification of a novel class of inhibitor (i.e., an active-site metal ion chelator) that acts against DENV RdRp activity. DENV RdRp utilizes a two-metal-ion mechanism of catalysis; therefore, we constructed a small library of compounds, th...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1994
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-75-8-1883